Coding
Part:BBa_K1903019:Design
Designed by: Rafael Montenegro Marín Group: iGEM16_TEC-Costa_Rica (2016-10-17)
Intein N SSp DnaB S1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence needs a reverse translate to the organism of interest.
The sequence needs an ATG codon to start transcription if wanted to express itself apart.
Source
The sequence of amino acids: LRESGCISGDSLISLA was reverse translate to E. coli
The amino acid sequence was obtained from: Volkmann, G., Volkmann, V., & Liu, X. Q. (2012). Site‐specific protein cleavage in vivo by an intein‐derived protease. FEBS letters, 586(1), 79-84.