Coding

Part:BBa_K1903019:Design

Designed by: Rafael Montenegro Marín   Group: iGEM16_TEC-Costa_Rica   (2016-10-17)


Intein N SSp DnaB S1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence needs a reverse translate to the organism of interest.

The sequence needs an ATG codon to start transcription if wanted to express itself apart.


Source

The sequence of amino acids: LRESGCISGDSLISLA was reverse translate to E. coli

The amino acid sequence was obtained from: Volkmann, G., Volkmann, V., & Liu, X. Q. (2012). Site‐specific protein cleavage in vivo by an intein‐derived protease. FEBS letters, 586(1), 79-84.

References